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Negative stain

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  • Negative stain — Electron micrograph of (organic) plant tissue without (top) and with OsO4 staining Negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. For bright field… …   Wikipedia

  • negative staining — n a method of demonstrating the form of small objects (as bacteria) by surrounding them with a stain that they do not take up so that they appear as sharply outlined unstained bright bodies on a colored ground * * * staining of the background and …   Medical dictionary

  • stain — 1. To discolor. 2. To color; to dye. 3. A discoloration. 4. A dye used in histologic and bacteriologic technique. 5. A procedure in which a dye or combination of dyes and reagents is used to color the constituents of cells and tissues. For… …   Medical dictionary

  • Stain, Gram — The Danish bacteriologist J.M.C. Gram (1853 1938) devised a method of staining bacteria using a dye called crystal (gentian) violet. Gram s method helps distinguish between different types of bacteria. The gram staining characteristics of… …   Medical dictionary

  • negative staining — Microscopic technique in which the object stands out against a dark background of stain. For electron microscopy the sample is suspended in a solution of an electron dense stain such as sodium phosphotungstate and then sprayed onto a support grid …   Dictionary of molecular biology

  • negative staining — noun : a method of demonstrating the form of small objects (as bacteria) by surrounding them with a stain that they do not take up so that they appear as sharply outlined unstained bright bodies on a colored ground * * * negative staining, the… …   Useful english dictionary

  • Gram-negative — bacteria lose the crystal violet stain (and take the color of the red counterstain) in Gram s method of staining. This is characteristic of bacteria that have a cell wall composed of a thin layer of a particular substance (called peptidoglycan).… …   Medical dictionary

  • Gram stain — The Danish bacteriologist J.M.C. Gram (1853 1938) devised a method of staining bacteria using a dye called crystal (gentian) violet. Gram s method helps distinguish between different types of bacteria. The gram staining characteristics of… …   Medical dictionary

  • Gram's stain — or Gram stain gram n 1) a method for the differential staining of bacteria that involves fixing the bacterial cells to a slide and staining usu. with the basic dye crystal violet, treating with an iodine solution to fix the dye, washing with… …   Medical dictionary

  • Periodic acid-Schiff stain — Periodic acid Periodic acid Schiff (PAS) is a staining method used to detect glycogen and other polysaccharides in tissues. The reaction of periodic acid oxidizes the diol functional groups in glucose and other sugars, creating aldehydes that… …   Wikipedia

  • Gram stain — noun a staining technique used to classify bacteria; bacteria are stained with gentian violet and then treated with Gram s solution; after being decolorized with alcohol and treated with safranine and washed in water, those that retain the… …   Useful english dictionary